Deleting the Outlook OST (Offline Storage Table) file is possible without losing access to data, as it serves as a cache for mailbox content. However, upon deletion, Outlook will automatically recreate the OST file by synchronizing with the Exchange server or other mail servers. While this process allows continued access to data, it's crucial to note that any changes made during offline usage may be lost. Users should exercise caution, back up important data by saving OST to PST file format, and understand that the recreated OST file will reflect the current state of the mailbox, potentially leading to the loss of unsynchronized changes if not properly managed.